Center for Pathogen Genomics and Microbial Evolution
Providing specialized expertise in pathogen-specific sequence analysis and bioinformatics for emerging and ongoing infectious disease threats.
The development of next generation DNA sequencing approaches has revolutionized genomics. As these technologies have become more affordable, easier to use and widely available, there has been an explosion of genomic sequence generated with hundreds of thousands of bacterial, viral and fungal genome sequences currently available through public databases. The wealth of microbial sequence data available, however, has far outpaced expertise and tools for analysis of these data. Owing in part to the considerable biological diversity among microbes - including bacteria, fungi and viruses - microbial genome sequence analysis has been difficult to standardize. Often, analysis approaches are not “one-size-fits-all” and a technique that is successful for studying one organism may not be directly applicable to another. To fully leverage the power of these tools, diverse expertise is required across several fields including microbiology, infectious disease clinical care, epidemiology and computational bioinformatics.
When properly harnessed through an integrated pipeline from sample acquisition to sequencing and bioinformatic analysis, pathogen genomics and molecular epidemiology can define the determinants of pathogen transmission, pathogenesis and evolution and so inform best practices in clinical care and public health. Indeed, the essentiality of rapid and effective microbial sequence analysis has been underscored by the global COVID-19 pandemic. Novel and creative approaches to SARS-CoV-2 viral sequencing and analysis have been central to important discoveries throughout the pandemic that have dictated diagnostic paradigms, facilitated drug discovery, charted routes of transmission and helped define best public health practices.
Northwestern has unmatched abilities in this field that is critical to the national and global efforts to track and study infectious disease threats. Implementing and expanding microbial genomic sequencing and innovative analysis capabilities pays dividends in infections prevented and lives saved.”
–Egon A. Ozer, MD, PhD, Director, Center for Pathogen Genomics and Microbial Evolution
Our Mission and Vision
Why It's Important
Microbial genomics: the study of the complete genetic code of pathogenic organisms
Center Leadership
Services
Featured Projects
SARS-CoV-2 Surveillance and Variant Tracking
Northwestern PIs: Egon A Ozer, MD, PhD; Judd F Hultquist, PhD
Early funding through the Dixon Family Foundation and the Northwestern University Clinical and Translational Sciences Institute supported the rapid establishment of SARS-CoV-2 sequencing within weeks of the first cases of COVID-19 diagnosed at Northwestern Memorial Hospital. This has led to ongoing genomic surveillance at Northwestern to perform tracking of emerging viral variants and study clinical impacts.
Surveillance and Transmission of SARS-CoV-2 Among Children in Chicago
Northwestern PIs: Larry K Kociolek, MD, MSCI
This project, led by researchers at Lurie Children’s Hospital and funded by the Walder Foundation, seeks to characterize and map potential SARS-CoV-2 transmissions among students, teachers, and school workers in classrooms where in-person instruction is occurring. It also tracks SARS-CoV-2 dynamics among pediatric infections in Chicago to study viral transmission and dynamics in children and adolescents.
The Global Threat of Antibiotic Resistance
Northwestern PIs: Mehreen Arshad, MBBS; Erica Hartmann PhD.
Infections caused by antibiotic-resistant bacteria are a growing threat around the world. This pilot study funded by the Northwestern Buffett Institute for Global Affairs seeks to develop an infrastructure to share clinical and pathogen genomic data as well as specimens from infections caused by antibiotic-resistant infections between institutions in the US and Pakistan. It also seeks to understand local and global factors influencing decision-making around antibiotic prescribing by healthcare providers.
Successful Clinical Response in Pneumonia Therapy (SCRIPT)
Northwestern PIs: Richard Wunderink, MD; Alan Hauser, MD, PhD
This U19 systems biology center funded by the NIAID investigates host/pathogen interactions that result in unsuccessful responses to therapy in severe pneumonia. The study combines clinical phenomics, host transcriptomics and epigenomics, pathogen genomics and alveolar metagenomics to model disease dynamics during ventilator-associated pneumonia.
Group A Streptococcus Molecular Epidemiology
Northwestern PIs: Ami Patel, MD, MPH; Judy Guzman-Cotrill, Robert Tanz, MD, Stan Shulman, MD
Infections with group A streptococci commonly cause can cause strep throat, but also severe invasive infections and post-infectious complications such as rheumatic fever. This CDC-supported project seeks to identify relationships between group A streptococci from across the United States and establish an active clinical and genomic surveillance network.
Role of HIV-1 Recombination in the Tissue Reservoir
Northwestern PIs: Richard T D'Aquila, MD; Ramon Lorenzo Redondo, PhD
This project through the Third Coast Center for AIDS Research (TC-CFAR) aims to develop a multidisciplinary experimental approach to study HIV-1 recombination levels in gastrointestinal tissue explant infection experiments. We plan to optimize this methodology to systematically and directly examine the mechanisms behind diversity preservation in infected tissues.
Phylodynamics and molecular epidemiology of SARS-CoV-2 in Nigeria
Northwestern PIs: Ramon Lorenzo Redondo, PhD
Supported by a Global Health Research Catalyzer Award, this study aims to study SARS-CoV-2 population dynamics in Nigeria in the framework of the current global pandemic to inform public-health responses. Additionally, it examines the population evolution and viral genes under selective pressure as potential therapeutic or immune targets.
Advanced molecular and network data combination to understand the HIV-1 epidemic among youth in Nigeria
Northwestern PIs: Babafemi O Taiwo, MBBS; Ramon Lorenzo Redondo, PhD
This project through the NICHD combines the use of social network and viral phylogenetic data to obtain the most complete picture of the HIV-1 epidemic in the young men who have sex with men (YMSM) population that Intensive Combination Approach to Rollback the Epidemic (iCARE) in Nigerian Adolescents is engaging in Nigeria.
Evolution of SARS-CoV-2 and Other Respiratory Viruses in Hospitalized Adults
Northwestern PIs: Michael G Ison, MD MS
This study aims to define the evolution of respiratory viruses including SARS-CoV-2, influenza, and RSV over time in isolates derived from hospital adults. Information about viral evolution over the course of respiratory infection combined with clinical data can provide insight into global evolutionary trends and host and viral responses and adaptations to infection.
Surveillance of antimicrobial-resistant Gram negative bacteria and fungi in a Pakistan health care system
Surveillance of antimicrobial-resistant Gram negative bacteria and fungi in a Pakistan health care system - CDC
Northwestern Faculty: PI Egon Ozer, MD, PhD
This project is focused on improving surveillance of infections with the antifungal-resistant yeast Candida auris in Pakistan through capacity-building for whole-genome sequencing and bioinformatic analysis.